Journal: Autophagy Reports
Article Title: Autophagy cargo profiles in skeletal muscle during starvation and exercise
doi: 10.1080/27694127.2025.2593060
Figure Lengend Snippet: Mitophagy and ribophagy in skeletal muscles under basal, starvation and exercise conditions. (A) (B) Mitophagy levels in basal, starvation and exercise conditions assessed in the muscles of Mito-QC mice. The numbers of red-only dots reflect the mitophagy flux and were quantified in (B). Data points are individual mice ( n = 4). * p < 0.05 using two sides, unpaired Student’s t-test when comparing with basal condition. & p < 0.05 using two sides, unpaired Student’s t-test when comparing with starvation condition. Data are mean ± s.e.m. Scale bar, 20 μm. (C) (D) Ribophagy levels in basal, starvation and exercise conditions assessed by examining the colocalization of ribosome protein S6 (RPS6) with lysosome protein LAMP1 in the muscles of wild-type mice. The numbers of colocalization dots per muscle fiber were quantified in (D). Data points are individual mice ( n = 3), and 40 to 60 fibers were quantified for each mouse. * p < 0.05 using two sides, unpaired Student’s t-test when comparing with basal condition. & p < 0.05 using two sides, unpaired Student’s t-test when comparing with exercise condition. Data are mean ± s.e.m. Scale bar, 10 μm. Arrowheads denote the colocalization dots.
Article Snippet: Anti-COXIV (11242–1-AP), anti-GAPDH (60004–1-Ig), anti-GM130 (11308–1-AP), anti-Cathepsin D (21327–1-AP), anti-ATP1A2 (16836–1-AP), anti-mouse LAMP1 (65050–1-Ig), anti-FAM134B (21537–1-AP) and anti-TEX264 (25858–1-AP) antibodies were from Proteintech.
Techniques: Muscles